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alcian blue stain kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories alcian blue stain kit
    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Alcian Blue Stain Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alcian+blue+stain+kit/Alcian+Blue+(pH+2%2E5)+Stain+Kit/pmc13114135-135-19-27
    Average 95 stars, based on 95 article reviews
    alcian blue stain kit - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis"

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

    Journal: Gut Microbes

    doi: 10.1080/19490976.2026.2662638

    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Figure Legend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Techniques Used: Staining, Western Blot, Quantitative RT-PCR, Expressing

    Related Articles

    Staining:

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis
    Article Snippet: Stained sections were imaged using VS120 Virtual Slide Microscope (Olympus) through the OlyVIA software. .. Alcian blue staining was performed to visualize acidic mucopolysaccharides and glycosaminoglycans in goblet cells of colon sections using the Alcian Blue Stain Kit (pH 2.5, Cat# H-3501, Vector Laboratories), following the manufacturer’s instructions. ..

    Article Title: Tumor microenvironment modulation by SERPINE1 increases radioimmunotherapy in murine model of gastric cancer
    Article Snippet: The slides were visualized, and images were taken with an Olympus BX53 system microscope (Olympus, Life Science Solution, Waltham, MA, USA). .. ECM polysaccharide staining was performed using an Alcian blue stain kit (Vector Laboratories, Burlingame, CA 94,010, Alcian Blue (pH 2.5), Stain kit, H-3501). ..

    Article Title: MIIST305 mitigates gastrointestinal acute radiation syndrome injury and ameliorates radiation-induced gut microbiome dysbiosis
    Article Snippet: .. Dewaxed and rehydrated colonic tissue samples were stained using the Alcian blue Stain Kit (Vector Laboratories, Newark, CA) according to the manufacturer’s protocol and examined microscopically using a 10X objective. ..

    Article Title: Tumor microenvironment modulation by SERPINE1 increases radioimmunotherapy in murine model of gastric cancer.
    Article Snippet: The slides were visualized, and images were taken with an Olympus BX53 system microscope (Olympus, Life Science Solution, Waltham, MA, USA). .. ECM polysaccharide staining was performed using an Alcian blue stain kit (Vector Laboratories, Burlingame, CA 94,010, Alcian Blue (pH 2.5), Stain kit, H-3501). ..

    Article Title: Deficiency of interleukin-1 receptor antagonist aggravates Citrobacter rodentium infection in mice
    Article Snippet: DuoSet enzyme-linked immunosorbent assay (ELISA) kits for mouse Lcn2, serum amyloid A (SAA), IL-1RA, IL-1β, and myeloperoxidase (MPO) were obtained from R&D Systems. .. Alcian blue stain kit was obtained from Vector Laboratories. .. Mouse lamina propria dissociation kit was purchased from Miltenyi Biotec.

    Article Title: A MTA2-SATB2 chromatin complex restrains colonic plasticity toward small intestine by retaining HNF4A at colonic chromatin.
    Article Snippet: Alkaline phosphatase enzyme was detected by Stemgent AP staining kit 2 (Pink color). .. An Alcian Blue Stain Kit (Vector Laboratories, H-3501) was used to stain goblet cells. ..

    Article Title: MIIST305 mitigates gastrointestinal acute radiation syndrome injury and ameliorates radiation-induced gut microbiome dysbiosis
    Article Snippet: .. Dewaxed and rehydrated colonic tissue samples were stained using the Alcian Blue Stain Kit (Vector Laboratories, Newark, CA) according to the manufacturer’s protocol and examined microscopically at (10 x objective) . ..

    Article Title: Promoting nutrient absorption through the colon
    Article Snippet: .. An Alcian Blue Stain Kit (Vector Laboratories, H-3501) was used to stain goblet cells. ..



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    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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    Image Search Results


    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Gut Microbes

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

    doi: 10.1080/19490976.2026.2662638

    Figure Lengend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: Alcian blue staining was performed to visualize acidic mucopolysaccharides and glycosaminoglycans in goblet cells of colon sections using the Alcian Blue Stain Kit (pH 2.5, Cat# H-3501, Vector Laboratories), following the manufacturer’s instructions.

    Techniques: Staining, Western Blot, Quantitative RT-PCR, Expressing

    V. parvula exacerbates colonic mucus barrier damage in DSS-induced colitis mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A,B) AB-PAS staining in mouse colon. (C) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). Data are expressed as mean ± S.D. (n = 6). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the DSV group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS group.

    Journal: Frontiers in Pharmacology

    Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

    doi: 10.3389/fphar.2026.1803816

    Figure Lengend Snippet: V. parvula exacerbates colonic mucus barrier damage in DSS-induced colitis mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A,B) AB-PAS staining in mouse colon. (C) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). Data are expressed as mean ± S.D. (n = 6). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the DSV group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS group.

    Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

    Techniques: Staining

    V. parvula exacerbates colonic barrier damage in DSS-induced colitis pseudo-germ-free mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A) The AB-PAS staining in mouse colon. (B) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). (C) The Western blot of mouse colon. The data are expressed as the means ± S.D. (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the VD group.

    Journal: Frontiers in Pharmacology

    Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

    doi: 10.3389/fphar.2026.1803816

    Figure Lengend Snippet: V. parvula exacerbates colonic barrier damage in DSS-induced colitis pseudo-germ-free mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A) The AB-PAS staining in mouse colon. (B) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). (C) The Western blot of mouse colon. The data are expressed as the means ± S.D. (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the VD group.

    Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

    Techniques: Staining, Western Blot